Journal: ACS applied materials & interfaces
Article Title: Caspase‑1 Responsive Nanoreporter for In Vivo Monitoring of Inflammasome Immunotherapy
doi: 10.1021/acsami.3c15733
Figure Lengend Snippet: Single nanoparticle activity analysis. (A) Representative confocal images of LPS primed iBMDMs treated with fluorescent nanoparticles encapsulated with FAM-tagged peptide (without quencher) at indicated concentrations ranging from 100 nM to 10 μM. The nuclei were stained with NucBlue (shown in blue). Scale bar: 200 mm. (B) Graph represents the percentage shift in fluorescence intensity of FAM by iBMDMs after 4 h of treatment with different concentrations of fluorescent Np. Data was acquired by flow cytometry. (C) Schematics representing the treatment sequence and specific time points for treatment of iBMDMs with MCC Np/FD, in order to determine their activity. (D) Graph indicates IL-1β levels in supernatant of nigericin-treated cells pretreated with either MCC-950 free drug or nanoparticle for 10 h. NT refers to no treatment with FD/Np. Data shown are mean ± s.e.m. (n = 3). Statistical analysis was performed by two-way ANOVA and Dunnett’s multiple comparisons test. FD and Np were compared using Sidak’s multiple comparisons test. ***p < 0.001,****p < 0.0001. (E) Graph displays percentage cytotoxicity induced from MCC-950 free drug or nanoparticle in primed iBMDMs after 10 h treatment. Data shown are mean ± s.e.m. (n = 3). Statistical analysis was performed by one-way ANOVA and Dunnett’s multiple comparisons test. **p < 0.01,****p < 0.0001.
Article Snippet: ProLong Diamond Antifade Mountant with DAPI (cat. no. P36962 ), NucBlue Live Ready Probes Reagent (Hoechst 33342), Lysotracker Red DND-99 (cat. no. L7528), and IL-1 β mouse uncoated ELISA kits (cat. no. 88–7013–88) were obtained from ThermoFisher Scientific.
Techniques: Activity Assay, Staining, Fluorescence, Flow Cytometry, Sequencing